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poly adp ribose par polymer  (Trevigen)

 
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    Trevigen poly adp ribose par polymer
    Poly Adp Ribose Par Polymer, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/poly+adp+ribose+par+polymer/pmc13137795-392-1-8?v=Trevigen
    Average 86 stars, based on 1 article reviews
    poly adp ribose par polymer - by Bioz Stars, 2026-07
    86/100 stars

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    The effect of 25-HC on AMPK signaling. (A) C57/B6 mice were intraperitoneally injected with 25-HC in IR models, and the left ventricular tissues were subjected to Western blot assay with anti-p38, JNK, and ERK antibodies. (B) Representative statistical results in each protein. (C) C57/B6 mice were intraperitoneally injected with 25-HC in IR models; the left ventricular tissues were subjected to Western blot assay with <t>anti-PAR</t> antibody. (D) The statistical relative expression level of <t>poly(ADP-ribosyl)ated</t> protein. Mean ± SEM. N = 5-6 per group. *P < 0.05 vs. vehicle group, #P < 0.01 vs. sham group.
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    The effect of 25-HC on AMPK signaling. (A) C57/B6 mice were intraperitoneally injected with 25-HC in IR models, and the left ventricular tissues were subjected to Western blot assay with anti-p38, JNK, and ERK antibodies. (B) Representative statistical results in each protein. (C) C57/B6 mice were intraperitoneally injected with 25-HC in IR models; the left ventricular tissues were subjected to Western blot assay with <t>anti-PAR</t> antibody. (D) The statistical relative expression level of <t>poly(ADP-ribosyl)ated</t> protein. Mean ± SEM. N = 5-6 per group. *P < 0.05 vs. vehicle group, #P < 0.01 vs. sham group.
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    The effect of 25-HC on AMPK signaling. (A) C57/B6 mice were intraperitoneally injected with 25-HC in IR models, and the left ventricular tissues were subjected to Western blot assay with anti-p38, JNK, and ERK antibodies. (B) Representative statistical results in each protein. (C) C57/B6 mice were intraperitoneally injected with 25-HC in IR models; the left ventricular tissues were subjected to Western blot assay with anti-PAR antibody. (D) The statistical relative expression level of poly(ADP-ribosyl)ated protein. Mean ± SEM. N = 5-6 per group. *P < 0.05 vs. vehicle group, #P < 0.01 vs. sham group.

    Journal: International Journal of Biological Sciences

    Article Title: 25-Hydroxycholesterol protects against myocardial ischemia-reperfusion injury via inhibiting PARP activity

    doi: 10.7150/ijbs.35075

    Figure Lengend Snippet: The effect of 25-HC on AMPK signaling. (A) C57/B6 mice were intraperitoneally injected with 25-HC in IR models, and the left ventricular tissues were subjected to Western blot assay with anti-p38, JNK, and ERK antibodies. (B) Representative statistical results in each protein. (C) C57/B6 mice were intraperitoneally injected with 25-HC in IR models; the left ventricular tissues were subjected to Western blot assay with anti-PAR antibody. (D) The statistical relative expression level of poly(ADP-ribosyl)ated protein. Mean ± SEM. N = 5-6 per group. *P < 0.05 vs. vehicle group, #P < 0.01 vs. sham group.

    Article Snippet: Then, they were transferred on polyvinylidene fluoride membranes (Millipore, USA), followed by overnight incubation with the following primary antibodies: P38 antibody (1:1000, Proteintech), JNK antibody (1:1000, Proteintech), ERK1/2 antibody (1:1000, Proteintech), phospho-p38 MAPK antibody (1:1000, CST), phospho-SAPK/JNK antibody (1:1000, CST), phospho-p44/p42 MAPK (Erk1/2) antibody (1:1000, CST), Bax antibody (1:1000, CST), BCL2 antibody (1:1000, CST), caspase-3 antibody (1:1000, Proteintech), cleaved caspase-3 antibody (1:1000, CST), poly(ADP-ribose) polymerase1 (parp1) antibody (1:1000, CST), GAPDH antibody (1:10000, Abcam), and anti-poly(ADP-ribose) polymer (PAR) antibody (1:1000, CST).

    Techniques: Injection, Western Blot, Expressing

    The PARP activity was inhibited by 25-HC in vitro and in vivo . (A) Nuclear extracts from primary mice myocardiocytes were incubated with different concentrations of 25-HC and then subjected to Western blot assay with anti-PAR antibody. (B) Neonatal C57BL/6 murine ventricular myocytes were subjected to H 2 O 2 (300 μmol/L) stimulation to measure protein level of PAR.

    Journal: International Journal of Biological Sciences

    Article Title: 25-Hydroxycholesterol protects against myocardial ischemia-reperfusion injury via inhibiting PARP activity

    doi: 10.7150/ijbs.35075

    Figure Lengend Snippet: The PARP activity was inhibited by 25-HC in vitro and in vivo . (A) Nuclear extracts from primary mice myocardiocytes were incubated with different concentrations of 25-HC and then subjected to Western blot assay with anti-PAR antibody. (B) Neonatal C57BL/6 murine ventricular myocytes were subjected to H 2 O 2 (300 μmol/L) stimulation to measure protein level of PAR.

    Article Snippet: Then, they were transferred on polyvinylidene fluoride membranes (Millipore, USA), followed by overnight incubation with the following primary antibodies: P38 antibody (1:1000, Proteintech), JNK antibody (1:1000, Proteintech), ERK1/2 antibody (1:1000, Proteintech), phospho-p38 MAPK antibody (1:1000, CST), phospho-SAPK/JNK antibody (1:1000, CST), phospho-p44/p42 MAPK (Erk1/2) antibody (1:1000, CST), Bax antibody (1:1000, CST), BCL2 antibody (1:1000, CST), caspase-3 antibody (1:1000, Proteintech), cleaved caspase-3 antibody (1:1000, CST), poly(ADP-ribose) polymerase1 (parp1) antibody (1:1000, CST), GAPDH antibody (1:10000, Abcam), and anti-poly(ADP-ribose) polymer (PAR) antibody (1:1000, CST).

    Techniques: Activity Assay, In Vitro, In Vivo, Incubation, Western Blot

    (A) and (B) PKO mice and C57/B6 mice were intraperitoneally injected with 25-HC in IR models, and the left ventricular tissues were subjected to Western blot assay with anti-PAR antibody. (C) PKO mice were intraperitoneally injected with 25-HC in IR models, and the left ventricular tissues were subjected to Western blot assay with anti-p38, JNK, and ERK antibodies. (D) Representative statistical results in each protein. Mean ± SEM. N = 5-6 per group.

    Journal: International Journal of Biological Sciences

    Article Title: 25-Hydroxycholesterol protects against myocardial ischemia-reperfusion injury via inhibiting PARP activity

    doi: 10.7150/ijbs.35075

    Figure Lengend Snippet: (A) and (B) PKO mice and C57/B6 mice were intraperitoneally injected with 25-HC in IR models, and the left ventricular tissues were subjected to Western blot assay with anti-PAR antibody. (C) PKO mice were intraperitoneally injected with 25-HC in IR models, and the left ventricular tissues were subjected to Western blot assay with anti-p38, JNK, and ERK antibodies. (D) Representative statistical results in each protein. Mean ± SEM. N = 5-6 per group.

    Article Snippet: Then, they were transferred on polyvinylidene fluoride membranes (Millipore, USA), followed by overnight incubation with the following primary antibodies: P38 antibody (1:1000, Proteintech), JNK antibody (1:1000, Proteintech), ERK1/2 antibody (1:1000, Proteintech), phospho-p38 MAPK antibody (1:1000, CST), phospho-SAPK/JNK antibody (1:1000, CST), phospho-p44/p42 MAPK (Erk1/2) antibody (1:1000, CST), Bax antibody (1:1000, CST), BCL2 antibody (1:1000, CST), caspase-3 antibody (1:1000, Proteintech), cleaved caspase-3 antibody (1:1000, CST), poly(ADP-ribose) polymerase1 (parp1) antibody (1:1000, CST), GAPDH antibody (1:10000, Abcam), and anti-poly(ADP-ribose) polymer (PAR) antibody (1:1000, CST).

    Techniques: Injection, Western Blot